Identification of Transcription Factors Involved in Rice Secondary Cell Wall Formation

Hirano, Ko; Kondo, Mari; Aya, Koichiro; Miyao, Akio; Sato, Yutaka; Antonio, Baltazar A.; Namiki, Nobukazu; Nagamura, Yoshiaki; Matsuoka, Makoto
November 2013
Plant & Cell Physiology;Nov2013, Vol. 54 Issue 11, p1791
Academic Journal
Using co-expression network analysis, we identified 123 transcription factors (TFs) as candidate secondary cell wall regulators in rice. To validate whether these TFs are associated with secondary cell wall formation, six TF genes belonging to the MYB, NAC or homeodomain-containing TF families were overexpressed or downregulated in rice. With the exception of OsMYB58/63-RNAi plants, all transgenic plants showed phenotypes possibly related to secondary cell wall alteration, such as dwarfism, narrow and dark green leaves, and also altered rice cinnamyl alcohol dehydrogenase 2 (OsCAD2) gene expression and lignin content. These results suggest that many of the 123 candidate secondary cell wall-regulating TFs are likely to function in secondary cell wall formation in rice. Further analyses were performed for the OsMYB55/61 and OsBLH6 TFs, the former being a TF in which the Arabidopsis ortholog is known to participate in lignin biosynthesis (AtMYB61) and the latter being one for which no previous involvement in cell wall formation has been reported even in Arabidopsis (BLH6). OsMYB55/61 and OsBLH6–GFP fusion proteins localized to the nucleus of onion epidermal cells. Moreover, expression of a reporter gene driven by the OsCAD2 promoter was enhanced in rice calli when OsMYB55/61 or OsBLH6 was transiently expressed, demonstrating that they function in secondary cell wall formation. These results show the validity of identifying potential secondary cell wall TFs in rice by the use of rice co-expression network analysis.


Related Articles

  • Regulation of the α-expansin gene OsEXPA8 expression affects root system architecture in transgenic rice plants. Wang, Ying; Ma, Nana; Qiu, Shichun; Zou, Hanyan; Zang, Guangchao; Kang, Zhenhui; Wang, Guixue; Huang, Junli // Molecular Breeding;Jun2014, Vol. 34 Issue 1, p47 

    Expansins are cell wall proteins implicated in the control of plant growth via loosening of the extracellular matrix, and are encoded by a large gene family. However, data linked to loss of function of single genes which support the role of expansins in root growth remain limited. In this study,...

  • OsGL1-3 is Involved in Cuticular Wax Biosynthesis and Tolerance to Water Deficit in Rice. Zhou, Xiaoyun; Li, Linzhi; Xiang, Jianhua; Gao, Guofu; Xu, Faxi; Liu, Ailing; Zhang, Xianwen; Peng, Yan; Chen, Xinbo; Wan, Xiangyuan // PLoS ONE;Jan2015, Vol. 10 Issue 1, p1 

    Cuticular wax covers aerial organs of plants and functions as the outermost barrier against non-stomatal water loss. We reported here the functional characterization of the Glossy1(GL1)-homologous gene OsGL1-3 in rice using overexpression and RNAi transgenic rice plants. OsGL1-3 gene was...

  • Engineering the Oryza sativa cell wall with rice NAC transcription factors regulating secondary wall formation. Kouki Yoshida; Shingo Sakamoto; Tetsushi Kawai; Yoshinori Kobayashi; Kazuhito Sato; Yasunori Ichinose; Katsuro Yaoi; Miho Akiyoshi-Endo; Hiroko Sato; TadashiTakamizo; Masaru Ohme-Takagi; Nobutaka Mitsuda // Frontiers in Plant Science;Oct2013, Vol. 4, p1 

    Plant tissues that require structural rigidity synthesize a thick, strong secondary cell wall of lignin, cellulose and hemicelluloses in a complicated bridged structure. Master regulators of secondary wall synthesis were identified in dicots, and orthologs of these regulators have been...

  • Cloning and Functional Analysis of a Novel DREB1/CBF Transcription Factor Involved in Cold-Responsive Gene Expression in Zea mays L. Feng Qin; Sakuma, Yoh; Jie Li; Qiang Liu; Yi-Qin Li; Shinozaki, Kazuo; Yamaguchi-Shinozaki, Kazuko // Plant & Cell Physiology;Aug2004, Vol. 45 Issue 8, p1042 

    The transcription factors DREB1s/CBFs specifically interact with the DRE/CRT cis-acting element (core motif: G/ACCGAC) and control the expression of many stress-inducible genes in Arabidopsis. We isolated a cDNA for a DREB1/CBF homolog, ZmDREB1A in maize using a yeast one-hybrid system. The...

  • OsbZIP71, a bZIP transcription factor, confers salinity and drought tolerance in rice. Liu, Citao; Mao, Bigang; Ou, Shujun; Wang, Wei; Liu, Linchuan; Wu, Yanbin; Chu, Chengcai; Wang, Xiping // Plant Molecular Biology;Jan2014, Vol. 84 Issue 1-2, p19 

    The bZIP transcription factor (TF) family plays an important role in the abscisic acid (ABA) signaling pathway of abiotic stress in plants. We here report the cloning and characterization of OsbZIP71, which encodes a rice bZIP TF. Functional analysis showed that OsbZIP71 is a nuclear-localized...

  • Expression of Arabidopsis DREB1C improves survival, growth, and yield of upland New Rice for Africa (NERICA) under drought. Ishizaki, Takuma; Maruyama, Kyonoshin; Obara, Mitsuhiro; Fukutani, Akiyo; Yamaguchi-Shinozaki, Kazuko; Ito, Yusuke; Kumashiro, Takashi // Molecular Breeding;Feb2013, Vol. 31 Issue 2, p255 

    Dehydration-responsive-element-binding protein 1 genes have important roles in response to stress. To improve the drought tolerance of an upland rice cultivar NERICA1, we introduced Arabidopsis AtDREB1C or rice OsDREB1B driven by a stress-inducible rice lip9 promoter. Plants of some transgenic...

  • Response of transgenic rape plants bearing the Osmyb4 gene from rice encoding a trans-factor to low above-zero temperature. Gomaa, A.; Raldugina, G.; Burmistrova, N.; Radionov, N.; Kuznetsov, Vl. // Russian Journal of Plant Physiology;Jan2012, Vol. 59 Issue 1, p105 

    Accumulation of soluble sugars (sucrose, fructose, and glucose), proline, phenols (total phenols and flavonoids), and antocyanins during adaptation to low-temperature stress (4°C) of two lines of spring rape ( Brassica napus L., cv. Westar) characterized by weak ( Bn-1) and strong ( Bn-3)...

  • The rice ERF transcription factor OsERF922 negatively regulates resistance to Magnaporthe oryzae and salt tolerance. Liu, Dongfeng; Chen, Xujun; Liu, Jiqin; Ye, Jianchun; Guo, Zejian // Journal of Experimental Botany;Oct2012, Vol. 63 Issue 10, p3899 

    Rice OsERF922, encoding an APETELA2/ethylene response factor (AP2/ERF) type transcription factor, is rapidly and strongly induced by abscisic acid (ABA) and salt treatments, as well as by both virulent and avirulent pathovars of Magnaporthe oryzae, the causal agent of rice blast disease....

  • Analysis of five novel putative constitutive gene promoters in transgenic rice plants. Su-Hyun Park; Nari Yi; Youn Shic Kim; Min-Ho Jeong; Seung-Woon Bang; Yang Do Choi; Ju-Kon Kim // Journal of Experimental Botany;Aug2010, Vol. 61 Issue 9, p2459 

    Novel constitutive gene promoters are essential components of crop biotechnology. Our analysis of five such promoters, APX, SCP1, PGD1, R1G1B, and EIF5, in transgenic rice plants is reported here. The five promoter regions were linked to the gfp reporter gene and transformed into rice. Using...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics