TITLE

Effect of Hydrostatic Pressure in The Presence of Staurosporine on Mouse Bone Marrow Mesenchymal Stem Cells

AUTHOR(S)
Javanmard, F.; Azadbakht, M.; Zhaleh, H.
PUB. DATE
June 2013
SOURCE
Cell Journal (Yakhteh);Summer 2013 Supplement 1, Vol. 15 Issue Sup 1, p48
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: Bone marrow mesenchymal stem cells (BMSCS) are multipotent, and proliferate freely in vitro to undergo self-renewal and differentiation into multiple nonhematopoietic cell lineages such as neuronal cells. The cells in the body are continuously exposed to a complex mechanical environment. Hydrostatic pressure as a mechanical force is effective in regulating the neural differentiation in many type of cell lines. The aim of the present study was to evaluate the effect of hydrostatic pressure in the presence of staurosporine on mouse bone marrow mesenchymal stem cells. Materials and Methods: Mouse mesenchymal stem cells are isolated from an aspirate of bone marrow harvested from the tibia and femoral marrow compartment. Cells cultured in DMEM culture medium supplemented with 10%FBS for 72 hours. After the first passage cells were cultured in treatment medium containing 100 nM of staurosporine for 4 h, than the cells were affected by hydrostatic pressure (0, 25, 50, 100 mm Hg). The cell viability and cell death were assessed using trypan blue and Hoechst/PI staining. Results: Our results indicate that the highest percentage of cell viability was 84% in the 0 mm Hg hydrostatic pressure. The lowest percentage of cell viability was 52 % in the 100 mm Hg hydrostatic pressure. The highest percentage of cell death was 52 % in the 100 mm Hg hydrostatic pressure after 24 hours and the lowest percentage of cell death was 17 % in the 0 mm Hg hydrostatic pressure. Conclusion: Based on these observations, we conclude that the viability of cells in treatments reduced according to increasing in hydrostatic pressure and with passing time. Our data revealed that the cell death in treatments increased according to increasing in hydrostatic pressure and with passing time.
ACCESSION #
93362913

 

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