TITLE

Effect of Hydrostatic Pressure in The Presence of Staurosporine on Neurite Outgrowth in Mouse Bone Marrow Mesenchymal Stem Cells

AUTHOR(S)
Javanmard, F.; Azadbakht, M.; Zhaleh, H.
PUB. DATE
June 2013
SOURCE
Cell Journal (Yakhteh);Summer 2013 Supplement 1, Vol. 15 Issue Sup 1, p49
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Objective: Bone marrow mesenchymal stem cells (BMSCS) are pluripotent cells that can differentiate into many types of cell lines including; neural cells. Mechanical forces are an important factor on nerve regeneration. It also should be noted that the mechanical stimulation could induce not only proliferation but also differentiation of MSCs. The cells in the body are continuously exposed to a complex mechanical environment. Hydrostatic pressure as a mechanical force, play a key role in regulating the neural differentiation in many type of cell lines. In this study we investigated the effect of hydrostatic pressure on neurite outgrowth and expression of neural markers in staurosporine-induced neural differentiation in mouse bone marrow mesenchymal stem cells. Materials and Methods: BMSCs were isolated from rat bone marrow based on their capacity to adhere to plastic culture surfaces and cultured in DMEM culture medium supplemented with 10% FBS. After the first passage cells were cultured in treatment medium containing 100 nM of staurosporine for 4 hours, than the cells were affected by hydrostatic pressure (0, 25, 50, 100 mmHg). The neurite outgrowth and expression of B-tubulin IIIand GFAP proteins was analysed by immunocytochemistry. Results: Our results showed that total neurite length were 178.89, 222.98, 170.17, 120.19 μm in 0, 25, 50, 100 mm Hg hydrostatic pressure treated cells; respectively. Total neurite length increase in 25 mm Hg hydrostatic pressure treated cells and decreased in 100 mm Hg hydrostatic pressure treated cells(p<0.05). Immunocytochemistry analysis revealed that expression ofB-tubulin III and GFAP proteins was strongly increased in 25 mm Hg hydrostatic pressure treated cells. Conclusion: According to our results, it is seem thathydrostatic pressure can improve length of neuritein mouse bone marrow mesenchymal stem cells in an amount dependent manner. Therefore hydrostatic pressureas a mechanical force can be used for improvement of neural differentiation in bone marrow mesenchymal stem cells.
ACCESSION #
93362914

 

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