The Effect of Cartilage Tissue Extraction of 13 Days-Old Mouse Embryo on Differentiation of Bone Marrow Mesenchymal Stem Cells to Osteoblasts

Ramezani, M.; Khanbabaee, R.; Hojati, V.
June 2013
Cell Journal (Yakhteh);Summer 2013 Supplement 1, Vol. 15 Issue Sup 1, p71
Academic Journal
Objective: Mesenchymal stem cells (MSCs) are nonhematopoietic and multipotent cells, which during development are derived from cells with a mesenchymal origin. Also they have high proliferative potential in the long term and are able to differentiation into osteoblast, osteocyte, adipocyte and condrocyte. In vitro osteoblastic differentiation needs some factors, which creates condition similar to bone tissue microenvironment. Materials and Methods: In this study, extract of cartilage tissue was studied to differentiation of bone marrow mesenchymal stem cell into osteoblast. Mesenchymal stem cells were isolated from mice bone marrow and cultured in flasks containing DMEM-F12, 100 μg/ml streptomycin, 100 U/ml penicillin and 10% FBS. Mesenchymal stem cells were purred by several passages; then, they were affected by mouse embryonic cartilage tissue extraction of 13 days old. After osteoblastic differentiation of mesenchymal stem cells, alizarin red staining was used to detect osteoblastic cells. Results: At the first, MSCs were spindle shape. After affect of extraction during 21 days, osteoblast cells and calcium crystals were observed. Conclusion: The results of the alizarin red staining shows the osteoblastic differentiation and also osteoblastic differentiation of mesenchymal stem cell confirmed that morphogens and osteogenic factors present in cartilage tissue extraction of 13 days old mouse embryo


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