Transdifferentiation of Adipose-Derived Stem Cells into Keratinocyte-Like Cells: Engineering a Stratified Epidermis

Chavez-Munoz, Claudia; Nguyen, Khang T.; Xu, Wei; Hong, Seok-Jong; Mustoe, Thomas A.; Galiano, Robert D.
December 2013
PLoS ONE;Dec2013, Vol. 8 Issue 12, p1
Academic Journal
Skin regeneration is an important area of research in the field of tissue-engineering, especially for cases involving loss of massive areas of skin, where current treatments are not capable of inducing permanent satisfying replacements. Human adipose-derived stem cells (ASC) have been shown to differentiate in-vitro into both mesenchymal lineages and non-mesenchymal lineages, confirming their transdifferentiation ability. This versatile differentiation potential, coupled with their ease of harvest, places ASC at the advancing front of stem cell-based therapies. In this study, we hypothesized that ASC also have the capacity to transdifferentiate into keratinocyte-like cells and furthermore are able to engineer a stratified epidermis. ASC were successfully isolated from lipoaspirates and cell sorted (FACS). After sorting, ASC were either co-cultured with human keratinocytes or with keratinocyte conditioned media. After a 14-day incubation period, ASC developed a polygonal cobblestone shape characteristic of human keratinocytes. Western blot and q-PCR analysis showed the presence of specific keratinocyte markers including cytokeratin-5, involucrin, filaggrin and stratifin in these keratinocyte-like cells (KLC); these markers were absent in ASC. To further evaluate if KLC were capable of stratification akin to human keratinocytes, ASC were seeded on top of human decellularized dermis and cultured in the presence or absence of EGF and high Ca2+ concentrations. Histological analysis demonstrated a stratified structure similar to that observed in normal skin when cultured in the presence of EGF and high Ca2+. Furthermore, immunohistochemical analysis revealed the presence of keratinocyte markers such as involucrin, cytokeratin-5 and cytokeratin-10. In conclusion this study demonstrates for the first time that ASC have the capacity to transdifferentiate into KLC and engineer a stratified epidermis. This study suggests that adipose tissue is potentially a readily available and accessible source of keratinocytes, particularly for severe wounds encompassing large surface areas of the body and requiring prompt epithelialization.


Related Articles

  • The Use of Human Sweat Gland-Derived Stem Cells for Enhancing Vascularization during Dermal Regeneration. Danner, Sandra; Kremer, Mathias; Petschnik, Anna Emilia; Nagel, Sabine; Zhang, Ziyang; Hopfner, Ursula; Reckhenrich, Ann K; Weber, Caroline; Schenck, Thilo L; Becker, Tim; Kruse, Charli; Machens, Hans-Günther; Egaña, José T // Journal of Investigative Dermatology;Jun2012, Vol. 132 Issue 6, p1707 

    Vascularization is a key process in tissue engineering and regeneration and represents one of the most important issues in the field of regenerative medicine. Thus, several strategies to improve vascularization are currently under clinical evaluation. In this study, stem cells derived from human...

  • Proliferation and Differentiation Potential of Human Adipose-Derived Stem Cells Grown on Chitosan Hydrogel. Debnath, Tanya; Ghosh, Sutapa; Potlapuvu, Usha Shalini; Kona, Lakshmi; Kamaraju, Suguna Ratnakar; Sarkar, Suprabhat; Gaddam, Sumanlatha; Chelluri, Lakshmi Kiran // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    Applied tissue engineering in regenerative medicine warrants our enhanced understanding of the biomaterials and its function. The aim of this study was to evaluate the proliferation and differentiation potential of human adipose-derived stem cells (hADSCs) grown on chitosan hydrogel. The...

  • Optimization of Parameters for a More Efficient Use of Adipose-Derived Stem Cells in Regenerative Medicine Therapies. Aguena, Meire; Fanganiello, Roberto Dalto; Lorico Tissiani, Luiz Alexandre; André Ishiy, Felipe Augusto; Atique, Rodrigo; Alonso, Nivaldo; Passos-Bueno, Maria Rita // Stem Cells International;2012, p1 

    Adipose tissue-derived stem cells (ASCs) association to fat in autologous lipotransfer is promising for a more effective soft tissue reconstruction, and optimization of protocols to isolate ASCs from lipoaspirate fat is much needed. We demonstrated that an increase in adipocyte differentiation...

  • Adipose-Derived Mesenchymal Stromal/Stem Cells: Tissue Localization, Characterization, and Heterogeneity. Baer, Patrick C.; Geiger, Helmut // Stem Cells International;2012, p1 

    Adipose tissue as a stem cell source is ubiquitously available and has several advantages compared to other sources. It is easily accessible in large quantities with minimal invasive harvesting procedure, and isolation of adipose-derived mesenchymal stromal/stem cells (ASCs) yields a high amount...

  • A Comparative Study of Osteogenic Differentiation Human Induced Pluripotent Stem Cells and Adipose Tissue Derived Mesenchymal Stem Cells. Ardeshirylajimi, Abdolreza; Soleimani, Masoud; Hosseinkhani, Saman; Parivar, Kazem; Yaghmaei, Parichehr // Cell Journal (Yakhteh);Autumn2014, Vol. 16 Issue 3, p235 

    Objective: Human induced pluripotent stem cells (iPSCs) have been shown to have promising capacity for stem cell therapy and tissue engineering applications. Therefore, it is essential to compare the ability of these cells with the commonly used mesenchymal stem cells (MSC) for bone tissue...

  • Neuronal Differentiation of Adipose Derived Stem Cells: Progress So Far. Moore, T. J.; Abrahamse, Heidi // International Journal of Photoenergy;2014, p1 

    The nervous system is essential for normal physiological function of all systems within the human body. Unfortunately the nervous system has a limited capacity for self-repair and there are a plethora of disorders, diseases, and types of trauma that affect the central and peripheral nervous...

  • The effects of etomidate and midazolam on adipose tissuederived mesenchymal stem cell proliferation. Ho Park; Kyoungho Ryu; Yun-Hong Kim; Won-Jun Choi; Dongchan Ko // Korean Journal of Anesthesiology;Dec2016, Vol. 69 Issue 6, p614 

    Background: Stem cell therapy using adipose tissue-derived mesenchymal stem cells (ADSCs), which are capable of multipotent differentiation, is currently being investigated in the field of tissue regeneration and the treatment of patients in intensive care units. It is known that type-A...

  • Parakeratotic Corneocytes Play a Unique Role in Human Skin Wound Healing. Usui, Marcia L; Underwood, Robert A; Fleckman, Philip; Olerud, John E // Journal of Investigative Dermatology;Mar2013, Vol. 133 Issue 3, p856 

    The article reflects on a study conducted to analyze the role played by parakeratotic corneocytes in human skin wound healing. The study examined the stratum lucidum interfollicular parakeratotic corneocytes (PCs) in the human skin wound specimens. The study found that PCs expand, migrate, and...

  • Hyaluronan Benzyl Ester as a Scaffold for Tissue Engineering. Vindigni, Vincenzo; Cortivo, Roberta; Iacobellis, Laura; Abatangelo, Giovanni; Zavan, Barbara // International Journal of Molecular Sciences;Jul2009, Vol. 10 Issue 7, p2972 

    Tissue engineering is a multidisciplinary field focused on in vitro reconstruction of mammalian tissues. In order to allow a similar three-dimensional organization of in vitro cultured cells, biocompatible scaffolds are needed. This need has provided immense momentum for research on "smart...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics