Clinical Forms of Canine Visceral Leishmaniasis in Naturally Leishmania infantum–Infected Dogs and Related Myelogram and Hemogram Changes

Nicolato, Roney de Carvalho; Abreu, Raquel Trópia de; Roatt, Bruno Mendes; Aguiar-Soares, Rodrigo Dian de Oliveira; Reis, Levi Eduardo Soares; Carvalho, Maria das Graças; Carneiro, Cláudia Martins; Giunchetti, Rodolfo Cordeiro; Bouillet, Leoneide Erica Maduro; Lemos, Denise Silveira; Coura-Vital, Wendel; Reis, Alexandre Barbosa
December 2013
PLoS ONE;Dec2013, Vol. 8 Issue 12, p1
Academic Journal
Hematological analysis has limited applications for disease diagnosis in Leishmania infantum–infected dogs, but it can be very important in evaluating the clinical forms of the disease and in understanding the evolution of canine visceral leishmaniasis (CVL) pathogenesis. Recently, we demonstrated that alterations in leucopoiesis and erythropoiesis are related to clinical status and bone marrow parasite density in dogs naturally infected by L. infantum. To further characterize these alterations, we evaluated the association between the hematological parameters in bone marrow and peripheral blood alterations in groups of L. infantum–infected dogs: asymptomatic I (AD-I: serum negative/PCR+), asymptomatic II (AD-II: serum positive), oligosymptomatic (OD), and symptomatic (SD). Results were compared with those from noninfected dogs (NID). The SD group was found to present a decrease in erythropoietic lineage with concomitant reductions in erythrocytes, hemoglobin, and hematocrit parameters, resulting in anemia. The SD group also had increased neutrophils and precursors and decreased band eosinophils and eosinophils, leading to peripheral blood leucopenia. In the AD-II group, lymphocytosis occurred in both the peripheral blood and the bone marrow compartments. The SD group exhibited lymphocytosis in the bone marrow, with lymphopenia in the peripheral blood. In contrast, the AD-I group, showed no significant changes suggestive of CVL, presenting normal counts in bone marrow and peripheral blood. Our results showed for the first time that important changes in hematopoiesis and hematological parameters occur during ongoing CVL in naturally infected dogs, mainly in symptomatic disease. Taken together, our results based on myelogram and hemogram parameters enable better understanding of the pathogenesis of the anemia, lymphocytosis, and lymphopenia, as well as the leucopenia (eosinopenia and monocytopenia), that contribute to CVL prognosis.


Related Articles

  • Efficacy and tolerability of oleylphosphocholine (OlPC) in a laboratory model of visceral leishmaniasis. Fortin, Anny; Hendrickx, Sarah; Yardley, Vanessa; Cos, Paul; Jansen, Herwig; Maes, Louis // Journal of Antimicrobial Chemotherapy (JAC);Nov2012, Vol. 67 Issue 11, p2707 

    Objectives The alkylphospholipid oleylphosphocholine (OlPC) is a structural analogue of miltefosine and may represent a potential therapeutic backup for the treatment of visceral leishmaniasis (VL). This laboratory study compared the in vitro and in vivo activity profile of both OlPC and...

  • Seroconversion of sentinel chickens as a biomarker for monitoring exposure to visceral Leishmaniasis. Ribeiro Soares, Bárbara; Almeida Souza, Ana Paula; Brito Prates, Deboraci; De Oliveira, Camila I.; Barral-Netto, Manoel; Carlos Miranda, José; Barral, Aldina // Scientific Reports;8/9/2013, p1 

    Leishmania infantum chagasi causes visceral leishmaniasis (VL); it is transmitted by the sand fly Lutzomyia longipalpis that injects saliva and parasites into the host's skin during a blood meal. Chickens represent an important blood source for sand flies and their presence in the endemic area...

  • Immunobiology of visceral leishmaniasis. Kumar, Rajiv; Nylén, Susanne // Frontiers in Immunology;Jul2012, Vol. 3, p1 

    Visceral leishmaniasis (VL), commonly known as kala-azar, is caused by Leishmania donovani and Leishmania infantum (Leishmania chagasi in the Americas). These Leishmania species infect macrophages throughout the viscera, and parasites are typically found in the spleen, liver and bone marrow....

  • Survey of Wild and Domestic Mammals for Infection with Leishmania infantum following an Outbreak of Desert Zoonotic Visceral Leishmaniasis in Jiashi, People's Republic of China. Gao, Chun-hua; Wang, Jun-yun; Zhang, Song; Yang, Yue-tao; Wang, Yong // PLoS ONE;7/15/2015, Vol. 10 Issue 7, p1 

    In 2008 and 2009, an outbreak of desert-subtype zoonotic visceral leishmaniasis occurred in Jiashi county, Xinjiang, China. So far, no animal reservoir has been identified for this type of visceral leishmaniasis. Therefore, we surveyed the most common mammals (wild and domestic) for Leishmania...

  • Accuracy and reproducibility of a rapid chromatographic immunoassay for the diagnosis of canine visceral leishmaniasis in Brazil. Schubach, Edvar Y. P.; Figueiredo, Fabiano B.; Romero, Gustavo A. S. // Transactions of the Royal Society of Tropical Medicine & Hygiene;Sep2014, Vol. 108 Issue 9, p568 

    Background Visceral leishmaniasis is a major public health concern in Brazil and the domestic dog is the main source of infection. In this study, we aimed to evaluate the accuracy and reliability of a rapid chromatographic immunoassay based on a dual-path platform for the diagnosis of canine...

  • Canine leishmaniosis: in vitro efficacy of miltefosine and marbofloxacin alone or in combination with allopurinol against clinical strains of Leishmania infantum. Farca, Anna; Miniscalco, B.; Badino, P.; Odore, R.; Monticelli, P.; Trisciuoglio, A.; Ferroglio, E. // Parasitology Research;Jun2012, Vol. 110 Issue 6, p2509 

    Despite the availability of different therapeutic options, canine visceral leishmaniosis (CVL) remains a challenging disease to treat. Recently miltefosine has been registered for use in dogs, and different studies have demonstrated its leishmanicidal effect. Moreover, it has been suggested that...

  • Comparison of hematological aspects: Visceral leishmaniasis and healthy children. Bafghi, Ali Fattahi; Shahcheraghi, Seyed Hossein; Nematollahi, Sedigheh // Tropical Parasitology;Jul2015, Vol. 5 Issue 2, p133 

    The article presents a comparison of the hematological aspects of children with visceral leishmaniasis (VL) and healthy children. Topics include the symptoms of VL such as anemia, hepatosplenomegaly, and remittent fever, VL's causing agent called Leishmania infantum, and the systemic parasitic...

  • Multi-antigen print immunoassay (MAPIA)-based evaluation of novel recombinant Leishmania infantum antigens for the serodiagnosis of canine visceral leishmaniasis. de Oliveira, Isaac Queiroz; Araujo Silva, Rodrigo; Vergne Sucupira, Michel; da Silva, Edmilson Domingos; Barbosa Reis, Alexandre; Grimaldi Jr., Gabriel; Mothé Fraga, Deborah Bittencourt; Tavares Veras, Patrícia Sampaio // Parasites & Vectors;2015, Vol. 8 Issue 1, p909 

    Background Domestic dogs are the principal reservoir hosts of Leishmania infantum in regions where visceral leishmaniasis (VL) is endemic. Although serologic methods are frequently used for the screening of infected dogs, antibody-based tests require further assessment, due to lack of...

  • The Antileishmanial Potential of C-3 Functionalized Isobenzofuranones against Leishmania (Leishmania) Infantum Chagasi. Pereira, Wagner Luiz; de Souza Vasconcellos, Raphael; Mariotini-Moura, Christiane; Gomes, Rodrigo Saar; de Cássia Firmino, Rafaela; da Silva, Adalberto Manoel; Silva Júnior, Abelardo; Bressan, Gustavo Costa; Almeida, Márcia Rogéria; Crocco Afonso, Luís Carlos; Teixeira, Róbson Ricardo; Rangel Fietto, Juliana Lopes // Molecules;Dec2015, Vol. 20 Issue 12, p22435 

    Leishmaniases are diseases caused by protozoan parasites of the genus Leishmania. Clinically, leishmaniases range from cutaneous to visceral forms, with estimated global incidences of 1.2 and 0.4 million cases per year, respectively. The treatment of these diseases relies on multiple parenteral...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics