Endothelial Differentiation of Mouse Mesenchymal Stem Cells

Mihai, CENARIU; PALL, Emoke; PARLAPAN, Laura; GROZA, Ioan
July 2013
Bulletin of the University of Agricultural Sciences & Veterinary;2013, Vol. 70 Issue 2, p198
Academic Journal
The purpose of this study focused on the isolation of mesenchymal stem cells from bone marrow, in vitro cultivation and morphology assessment of isolated cells, morphological and phenotypic characterization and evaluation of multilinearity capability through differentiation of primary culture towards endothelial line. The biological material was represented by CD1 mice that were killed and the femur bone was collected under maximum sterility conditions. The cultivation of mesenchymal stem cells isolated from bone marrow was performed using the IMDM medium, supplemented with 10% FCS, 1% Antibiotic-Antimycotic, 1% glutamine, 1% NEA and 5% horse serum. Mesenchymal stem cell differentiation towards the endothelial line was induced using the EBM2 medium supplemented with EGM2 SingleQuots. The multilinearity of murine mesenchymal stem cells was demonstrated by flow cytometry, after differentiation, using anti CD34 and CD 105 (Endoglin) antibodies. The results proved that the EBM2 treated cultures supplemented with SingleQuots showed specific endothelial morphologic characteristics, 4% of them being positive for CD34, while 67.2 % were positive for CD105


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