A sensitive immunosorbent bio-barcode assay based on real-time immuno-PCR for detecting 3,4,3',4'-tetrachlorobiphenyl

Yang, Guang-Xin; Zhuang, Hui-Sheng; Chen, Han-Yu; Ping, Xian-Yin; Bu, Dan
February 2014
Analytical & Bioanalytical Chemistry;Feb2014, Vol. 406 Issue 6, p1693
Academic Journal
A functionalized gold-nanoparticle bio-barcode assay, based on real-time immuno-PCR (IPCR), was designed for the determination of 3,4,3',4'-tetrachlorobiphenyl (PCB77). 15 nm gold nanoparticles were synthesized, and modified with thiol-capped DNA and goat anti-rabbit IgG. The nanoparticle probes were used to replace antibody-DNA conjugate in the IPCR, and were fixed on the PCR tube wall via the immune reaction. Real-time PCR was performed to quantify the DNA signal directly. Under optimized conditions, the new method was used to detect PCB77 with a linearity range from 5 pg L to 10 ng L, and the limit of detection (LOD) was 1.72 pg L. Real samples of Larimichthys polyactis, collected from the East China Sea, were analyzed. Recovery was from 82 % to 112 %, and the coefficient of variation (CV) was acceptable. The results were compared with GC-ECD, revealing that the method would be acceptable for providing rapid, semi-quantitative, and reliable test results for making environmental decisions. [Figure not available: see fulltext.]


Related Articles

  • LABORATORY DIAGNOSIS AND CLINICAL SIGNS OF CANINE VISCERAL LEISHMANIASIS IN DOGS EXAMINED AT THE CENTER FOR ZOONOSIS CONTROL IN CAMPO GRANDE - MS, BRAZIL. Cunha, Rodrigo Casquero; Andreotti, Renato; Araujo e Silva, Elaine; Pereira, Elisângela; Sato, Tayra; Thomaz-Soccol, Vanete // Archives of Veterinary Science;2012, Vol. 17 Issue 4, p17 

    Visceral leishmaniasis is a type of zoonosis caused by several Leishmania species endemic to tropical, subtropical, and Mediterranean climate regions. Dogs are the primary source of infection in urban areas and can be symptomatic or asymptomatic. This study focused on the observation of clinical...

  • Current status of African swine fever virus in a population of wild boar in eastern Poland (2014-2015). Woźniakowski, Grzegorz; Kozak, Edyta; Kowalczyk, Andrzej; Łyjak, Magdalena; Pomorska-Mól, Małgorzata; Niemczuk, Krzysztof; Pejsak, Zygmunt // Archives of Virology;Jan2016, Vol. 161 Issue 1, p189 

    African swine fever virus (ASFV) was detected in wild boar in eastern Poland in early 2014. So far, 65 cases of ASFV infection in wild boar have been recognised. The methods used for ASFV detection included highly specific real-time PCR with a universal probe library (UPL), enzyme-linked...

  • Evaluation of the mineralizable organic nitrogen in soil using an immunological technique. Shingo Matsumoto; Noriharu Ae; Yohichi Koyama; Katsumasa Iijima; Rumiko Kodashima; Mari Hirata; Junko Kasuga; Nobuhisa Koga // Biology & Fertility of Soils;Oct2008, Vol. 45 Issue 1, p107 

    Abstract  Phosphate buffer-extractable organic nitrogen (PEON), which can be extracted from soil with a 67-mM neutral phosphate buffer solution, is considered to be a major source of mineralizable nitrogen (N) that is ubiquitous in soil. We have developed a Western blot analysis...

  • Lack of Evidence of Measles Virus Shedding in People with Inapparent Measles Virus Infections. Lievano, Fabio A.; Papania, Mark J.; Helfand, Rita F.; Harpaz, Rafael; Walls, Laura; Katz, Russell S.; Williams, Irene; Villamarzo, Yvonne S.; Rota, Paul A.; Bellini, William J. // Journal of Infectious Diseases;5/1/2004 Supplement, Vol. 189, pS165 

    Serological evidence of measles virus infection has been detected among people exposed to measles who do not exhibit classical clinical symptoms. Throat swabs, lymphocytes, and serum and urine samples were collected from contacts of individuals with confirmed measles 12-16 days after exposure,...

  • Single-chain antibody against human lipocalin-type prostaglandin D synthase: Construction, expression, purification, and activity assay. De-Yu Chen; Li-Min Liu; Sheng-Jie Liu; Mao-Ying Zhu; Lan Xu; Tian-Hua Huang // Biochemistry (00062979);Jun2008, Vol. 73 Issue 6, p702 

    An active form of single-chain antibody (ScFv) from murine monoclonal antibody 4A7, which is specific for lipocalin-type prostaglandin D synthase (L-PGDS), was produced in Escherichia coli. The complementary DNA fragments encoding the variable regions of heavy chain (VH) and light chain (VL),...

  • Comparison of PCR and antigen detection methods for diagnosis of Entamoeba histolytica infection. Furrows, S. J.; Moody, A. H.; Chiodini, P. L. // Journal of Clinical Pathology;Dec2004, Vol. 57 Issue 12, p1264 

    Aims: To assess different laboratory methods for the identification of Entamoeba histolytica in clinical samples. Methods: Antigen detection enzyme linked immunosorbent assay, polymerase chain reaction solution hybridisation enzyme linked immunoassay (PCR-SHELA), and a commercial Lightcycler PCR...

  • Novel Strategies and Tools for Enhanced Sensitivity in Routine Biomolecule Analytics. Adler, Michael; Spengler, Mark // Current Pharmaceutical Analysis;Nov2009, Vol. 5 Issue 4, p390 

    The combination of flexible and robust immunoassay technology with the sensitivity of DNA as a signal amplification template is realized in Immuno-PCR ("IPCR"). Classical ELISA is converted to IPCR by exchange of antibody-enzyme detection conjugates with antibody-DNA conjugates. The value of...

  • Real-time PCR demonstrates a higher prevalence of Mycoplasma bovis in Northern Ireland compared with sandwich ELISA. Bell, C. J.; Blackburn, P.; Patterson, I. A. P.; Ellison, S.; Ball, H. J. // Veterinary Record: Journal of the British Veterinary Association;10/20/2012, Vol. 171 Issue 16, preceding p402 

    The article offers information on the study conducted related to the prevalence of Mycoplasma bovis (M bovis) in Northern Ireland compared to sandwich enzyme-linked immunosorbent assay (ELISA). It states that Real-time polymerase chain reaction (qPCR) assays have been developed in recent years...

  • A Mumps Outbreak in Vojvodina, Serbia, in 2012 Underlines the Need for Additional Vaccination Opportunities for Young Adults. Nedeljković, Jasminka; Kovačević-Jovanović, Vesna; Milošević, Vesna; Šeguljev, Zorica; Petrovic, Vladimir; Muller, Claude P.; Hübschen, Judith M. // PLoS ONE;10/23/2015, Vol. 10 Issue 10, p1 

    In 2012, mumps was introduced from Bosnia and Herzegovina to Vojvodina, causing an outbreak with 335 reported cases. The present manuscript analyses the epidemiological and laboratory characteristics of this outbreak, identifies its main causes and suggests potential future preventive measures....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics