TITLE

Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells

AUTHOR(S)
Chan, Alexander; Heathman, Thomas; Coopman, Karen; Hewitt, Christopher
PUB. DATE
April 2014
SOURCE
Biotechnology Letters;Apr2014, Vol. 36 Issue 4, p731
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Extracellular surface proteins are used to identify fully-functional human mesenchymal stem cells (hMSCs) in a mixed population. Here, a multiparameter flow cytometry assay was developed to examine the expression of several bone marrow-derived hMSC markers simultaneously at the single cell level. The multiparameter approach demonstrates a depth of analysis that goes far beyond the conventional single or dual staining methods. CD73, CD90 and CD105 were chosen as positive markers as they are expressed on multipotent hMSCs, whilst CD34 and HLA-DR were chosen as negative indicators. Single colour analysis suggested a population purity of 100 %; in contrast, when analysed via the multiparameter method, the CD73/CD105/CD90/HLA-DR/CD34 phenotypes represented 94.5 ± 1.3 % of the total cell population. Also, although CD271 has been posited as a definite early stage hMSC marker, here we show it is not present on pre-passage cells, highlighting the need for careful marker selection.
ACCESSION #
94942117

 

Related Articles

  • MSC surface markers (CD44, CD73, and CD90) can identify human MSC-derived extracellular vesicles by conventional flow cytometry. Ramos, Teresa L.; Sánchez-Abarca, Luis Ignacio; Muntión, Sandra; Preciado, Silvia; Puig, Noemí; López-Ruano, Guillermo; Hernández-Hernández, Ángel; Redondo, Alba; Ortega, Rebeca; Rodríguez, Concepción; Sánchez-Guijo, Fermín; Cañizo, Consuelo del // Cell Communication & Signaling;1/12/2016, Vol. 14, p1 

    Background: Human mesenchymal stromal cells (hMSC) are multipotent cells with both regenerative and immunomodulatory activities making them an attractive tool for cellular therapy. In the last few years it has been shown that the beneficial effects of hMSC may be due to paracrine effects and, at...

  • Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions. Schirmaier, Carmen; Kaiser, Stephan C.; Jossen, Valentin; Brill, Silke; Jüngerkes, Frank; van den Bos, Christian; Eibl, Dieter; Eibl, Regine // BMC Proceedings;12/4/2013, p1 

    The article presents a study which discusses expansion of mesenchymal adipose-tissue derived stem cells in a single-use bioreactor under low-serum conditions. Topics discussed include increase in demand of human mesenchymal stem cells (hMSCs), use of preliminary tests in disposable-spinners to...

  • Nonadherent culture method downregulates stem cell antigen-1 expression in mouse bone marrow mesenchymal stem cells. BAOPING DENG; WEIPING DENG; PINGNAN XIAO; KUAN ZENG; SHINING ZHANG; HONGWU ZHANG; DAVID YB DENG; YANQI YANG // Experimental & Therapeutic Medicine;2015, Vol. 10 Issue 1, p31 

    Mesenchymal stem cells (MSCs) are primarily isolated by their adherence to plastic and their in vitro growth characteristics. Expansion of these cells from an adherent culture is the only method to obtain a sufficient number of cells for use in clinical practice and research. However, little is...

  • Endothelial Differentiation of Mouse Mesenchymal Stem Cells. Mihai, CENARIU; PALL, Emoke; PARLAPAN, Laura; GROZA, Ioan // Bulletin of the University of Agricultural Sciences & Veterinary;2013, Vol. 70 Issue 2, p198 

    The purpose of this study focused on the isolation of mesenchymal stem cells from bone marrow, in vitro cultivation and morphology assessment of isolated cells, morphological and phenotypic characterization and evaluation of multilinearity capability through differentiation of primary culture...

  • Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents. Riordan, Neil H.; Madrigal, Marialaura; Reneau, Jason; de Cupeiro, Kathya; Jiménez, Natalia; Ruiz, Sergio; Sanchez, Nelsy; Ichim, Thomas E.; Silva, Francisco; Patel, Amit N. // Journal of Translational Medicine;Jul2015, Vol. 13 Issue 1, p1 

    Background: The rapid clinical translation of mesenchymal stem cells (MSC) has resulted in the development of cell-based strategies for multiple indications. Unfortunately one major barrier to widespread implementation of MSCbased therapies is the limited supply of fetal calf serum (FCS) used to...

  • Nesprin-1 has key roles in the process of mesenchymal stem cell differentiation into cardiomyocyte-like cells in vivo and in vitro. WENGANG YANG; HUI ZHENG; YONGYI WANG; FENG LIAN; ZHENGLEI HU; SONG XUE // Molecular Medicine Reports;2015, Vol. 11 Issue 1, p133 

    The aim of the present study was to investigate the expression of nesprin-1 protein in MSCs and its effects on the differentiation of rat bone-marrow mesenchymal stem cells (MSCs). Surface-associated antigens of MSCs were detected by flow cytometry. MSC differentiation was induced by treatment...

  • Effects of lentiviral infection of mesenchymal stem cells on the expression of octamer transcription factor 4. JING CHANG; LI TANG; HAN LEI; XIAO-GANG ZHANG; ZHONG ZUO; WEI HUANG; HANG FU // Molecular Medicine Reports;2014, Vol. 10 Issue 5, p2249 

    The present study aimed to investigate the effects of lentiviral infection of human umbilical cord mesenchymal stem cells (hUCMSCs) on the expression of octamer transcription factor 4 (Oct4). hUCMSCs were infected with lentivirus carrying the green fluorescent protein gene (GFP) at different...

  • Characteristics and response of mouse bone marrow derived novel low adherent mesenchymal stem cells acquired by quantification of extracellular matrix. Ri-Cheng Zheng; Seong-Kyun Kim; Seong-Joo Heo; Jai-Young Koak; Joo-Hee Lee; Ji-Man Park // Journal of Advanced Prosthodontics;Oct2014, Vol. 6 Issue 5, p351 

    PURPOSE. The aim of present study was to identify characteristic and response of mouse bone marrow (BM) derived low-adherent bone marrow mesenchymal stem cells (BMMSCs) obtained by quantification of extracellular matrix (ECM). MATERIALS AND METHODS. Non-adherent cells acquired by ECM coated...

  • Effects of mesenchymal stromal cell-derived extracellular vesicles on tumor growth. Bruno, Stefania; Collino, Federica; Iavello, Alessandra; Camussi, Giovanni // Frontiers in Immunology;Jul2014, Vol. 5, preceding p1 

    Extracellular vesicles (EVs) are membrane vesicles, which are secreted by a variety of cells that have a relevant role in intercellular communication. EVs derived from various cell types exert different effects on target cells. Mesenchymal stromal cells (MSCs) are stem cells that are...

Share

Read the Article

Courtesy of NEW JERSEY STATE LIBRARY

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics