TITLE

Effects of methylthiouracil on the proliferation and apoptosis of rat bone marrow stromal cells

AUTHOR(S)
ZHONG-LU YE; XIAO-XIAO HOU; RE-LING CHEN; JIE DING; GUO-HUA ZHENG; MING-ZHEN CHEN; CHUAN TIAN
PUB. DATE
June 2014
SOURCE
Experimental & Therapeutic Medicine;2014, Vol. 7 Issue 6, p1738
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
The aim of the present study was to investigate the effects of methylthiouracil (MTU) on the proliferation and apoptosis of rat bone marrow stromal cells (BMSCs). Rat BMSCs were isolated, cultured in vitro and treated with various concentrations of MTU. Cell growth curves were determined using the Cell Counting Kit-8 method and the effect of MTU on BMSCs in a logarithmic growth phase was observed. BMSC apoptosis following MTU treatment was detected by flow cytometry. The experimental results demonstrated that the proliferation-inhibition effect was gradually enhanced with increasing MTU concentrations and the extension of treatment time. Statistically significant differences were observed between the treatment and the control groups (P<0.05). In addition, the BMSC apoptosis rate gradually increased with increasing drug concentrations and treatment time extension; statistically significant differences were observed between the treatment and the control groups (P<0.05). Therefore, the results of the present study demonstrated that MTU inhibited the proliferation of BMSCs and promoted apoptosis, indicating the cytotoxic effects of MTU on BMSCs.
ACCESSION #
95959428

 

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