Protection of mesenchymal stem cells on acute kidney injury

January 2014
Molecular Medicine Reports;2014, Vol. 9 Issue 1, p91
Academic Journal
The aim of the present study was to determine the protection of allogeneic bone marrow mesenchymal stem cells (BMSCs) on an ischaemia/reperfusion (I/R)-induced acute kidney injury (AKI) rat model and to investigate the underlying mechanisms. The BMSCs were isolated and cultured from adult Sprague-Dawley (SD) rats and the I/R-induced AKI model was established by bilateral clamping of renal pedicles for 60 min. Following successfully establishing the AKI model, 1x106 BMSCs were administered by intrarenal injection. All animals were randomly divided into four groups (n=10 in each): 1 (sham control), 2 (I/R), 3 (I/R + culture medium) and 4 (I/R + BMSCs). Serum levels of creatinine (Cr) and blood urea nitrogen (BUN) were measured in all four groups at 24 and 72 h. Three days post-surgery, the level of inflammatory factors, including interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) and vascular endothelial growth factor (VEGF) in the kidney was analysed by quantitative polymerase chain reaction. Three days following surgery, mRNA expression levels of IL-6 and TNF-α were significantly lower, however, the expression level of VEGF was significantly higher in group 4 compared with groups 2 and 3 (P<0.05). By contrast, the immunofluorescence results showed that the injected BMSCs differentiated into vascular endothelial cells. In conclusion, the present study identified that intrarenal administration of BMSCs improved I/R-induced AKI through the anti-inflammatory effect and a paracrine mechanism and therefore, may be hypothesised for the use in clinical trials.


Related Articles

  • Mesenchymal Stromal Cell-Derived Extracellular Vesicles Protect Against Acute Kidney Injury Through Anti-Oxidation by Enhancing Nrf2/ARE Activation in Rats. Zhang, Guangyuan; Zou, Xiangyu; Huang, Yeqing; Wang, Feng; Miao, Shuai; Liu, Guohua; Chen, Ming; Zhu, Yingjian // Kidney & Blood Pressure Research;Mar2016, Vol. 41 Issue 2, p119 

    Background/Aims: Anti-oxidation is an effective strategy for curing acute kidney injury (AKI). Herein, we suggest that extracellular vesicles (EVs) derived from mesenchymal stromal cells (MSCs) might play an anti-oxidative role by enhancing Nrf2/ARE activation in AKI. Methods: EVs isolated from...

  • Bone Marrow-Derived Mesenchymal Stem Cells Repaired but Did Not Prevent Gentamicin-Induced Acute Kidney Injury through Paracrine Effects in Rats. Reis, Luciana A.; Borges, Fernanda T.; Simões, Manuel J.; Borges, Andrea A.; Sinigaglia-Coimbra, Rita; Schor, Nestor; Camussi, Giovanni // PLoS ONE;Sep2012, Vol. 7 Issue 9, following p1 

    This study evaluated the effects of bone marrow-derived mesenchymal stem cells (BMSCs) or their conditioned medium (CM) on the repair and prevention of Acute Kidney Injury (AKI) induced by gentamicin (G). Animals received daily injections of G up to 20 days. On the 10th day, injections of BMSCs,...

  • Myogenic Potential of Whole Bone Marrow Mesenchymal Stem Cells In Vitro and In Vivo for Usage in Urinary Incontinence. Gunetti, Monica; Tomasi, Simone; Giammò, Alessandro; Boido, Marina; Rustichelli, Deborah; Mareschi, Katia; Errichiello, Edoardo; Parola, Maurizio; Ferrero, Ivana; Fagioli, Franca; Vercelli, Alessandro; Carone, Roberto; Câmara, Niels Olsen Saraiva // PLoS ONE;Sep2012, Vol. 7 Issue 9, Special section p1 

    Urinary incontinence, defined as the complaint of any involuntary loss of urine, is a pathological condition, which affects 30% females and 15% males over 60, often following a progressive decrease of rhabdosphincter cells due to increasing age or secondary to damage to the pelvic floor...

  • In vitro gene and chromosome characterization of expanded bone marrow mesenchymal stem cells for musculo-skeletal applications. ROSETI, L.; SERRA, M.; CANELLA, E.; MUNNO, C.; TOSI, A.; ZUNTINI, M.; PANDOLFI, M.; SANGIORGI, L.; BISO, R.; PITTALIS, M. C.; BINI, C.; PELOTTI, S.; GASBARRINI, A.; BORIANI, L.; BASSI, A.; GRIGOLO, B. // European Review for Medical & Pharmacological Sciences;Dec2014, Vol. 18 Issue 23, p3702 

    OBJECTIVE: A number of studies have shown the role of expanded Bone Marrow-derived Mesenchymal Stem Cells in the repair and regeneration of musculo-skeletal tissues. The current European regulations define in vitro expanded cells for clinical purposes as substantially manipulated and include...

  • Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells. First Successful Latin-American Report. Mardones, Rodrigo; Martínez, Rafael; Paredes, María Jose; Zalaquett, Eugenio // International Journal of Morphology;2010, Vol. 28 Issue 3, p749 

    Osteoarthritis is the more frequent cause of disability in adult people and it is associated to cartilage degeneration of affected joints. This cartilage has a limited ability to repair. Several treatments have been tested including the use of Mesenchymal Stem Cells. These cells are an...

  • In vitro differentiation of mesenchymal stem cells into endothelial cells. Siddiqui, Irfan Ahmed // Der Pharmacia Sinica;2013, Vol. 4 Issue 1, p106 

    The aim of the investigation was to detect the in vitro differentiation of mesenchymal stem cells (MSCs) into cells of the endothelial lineage. MSCs were generated from mononuclear bone marrow cells from healthy donors separated by density gradient centrifugation. Cells were characterized by...

  • Effect of TNF-α Inhibition on Bone Marrow-Derived Mesenchymal Stem Cells in Neurological Function Recovery after Spinal Cord Injury via the Wnt Signaling Pathway in a Rat Model. Peng, Ren-Jun; Jiang, Bing; Ding, Xi-Ping; Huang, He; Liao, Yi-Wei; Peng, Gang; Cheng, Quan; Xi, Jian // Cellular Physiology & Biochemistry (Karger AG);Jul2017, Vol. 42 Issue 2, p743 

    Aim: The present study aimed to examine the effect of tumor necrosis factor-α (TNF-α) inhibition on bone marrow-derived mesenchymal stem cells (BMSCs) in neurological function recovery after spinal cord injury (SCI) via the Wnt signaling pathway in a rat model. Methods: The rat model of...

  • Mesenchymal stem cells immunosuppressive properties: is it specific to bone marrow-derived cells? Jorgensen, Christian // Stem Cell Research & Therapy;2010, Vol. 1 Issue 2, p1 

    Based on their capacity to suppress immune responses, multipotent mesenchymal stromal cells (MSCs) are intensively studied for regenerative medicine. Moreover, MSCs have paracrine effects, including immunomodulation that occurs through the secretion of soluble mediators, including nitric oxide...

  • Survival and distribution of injected haematopoietic stem cells in acute kidney injury. Burst, Volker; Pütsch, Florian; Kubacki, Torsten; Völker, Linus A.; Bartram, Malte P.; Müller, Roman-Ulrich; Gillis, Meyke; Kurschat, Christine E.; Grundmann, Franziska; Müller-Ehmsen, Jochen; Benzing, Thomas; Teschner, Sven // Nephrology Dialysis Transplantation;May2013, Vol. 28 Issue 5, p1131 

    Background Endogenous bone marrow-derived cells are known to incorporate into renal epithelium at a low rate. Haematopoietic stem cells (HSCs) rather than mesenchymal stem cells (MSC) are responsible for this phenomenon. MSCs have the potential to ameliorate kidney function after acute kidney...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics