TITLE

Regulatory Role of CD4CD25Foxp3 Regulatory T Cells on IL-17-Secreting T Cells in Chronic Hepatitis B Patients

AUTHOR(S)
Li, Jie; Shi, Jun; Ren, Wanhua; Wu, Wei; Chen, Zhi
PUB. DATE
July 2014
SOURCE
Digestive Diseases & Sciences;Jul2014, Vol. 59 Issue 7, p1475
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Both interleukin (IL)-17-secreting CD4 T (Th17) and CD4CD25Foxp3 T regulatory (Treg) cells have been shown to be associated with disease progression or liver damage in chronic hepatitis B (CHB) patients. However, the relationship between Treg cells and IL-17-secreting T cells in hepatitis B virus (HBV) infections is unclear. Methods: The frequencies of Treg cells and IL-17-secreting T cells in hepatitis B e antigen (HBeAg)-positive CHB patients and healthy subjects were measured by flow cytometric analysis. The role of Treg cells on the differentiation of Ag-specific IL-17-secreting T cells was determined by removing the Treg cells from peripheral blood mononuclear cells (PBMCs) in HBeAg-positive CHB patients. Results: The frequencies of both Th17 (1.71 ± 0.58 vs. 1.08 ± 0.36 %; P < 0.01) and Treg cells (8.92 ± 4.11 vs. 6.45 ± 1.56 %; P < 0.01) were increased in the peripheral blood of HBeAg-positive CHB patients compared with healthy controls, but in not the IL-17-secreting CD8 T (Tc17) cells. The frequency of Treg cells was significantly associated with that of Th17 cells ( r = 0.625, P = 0.001) in CHB patients. Spearman analysis showed a positive correlation between the frequency of Treg cells and HBV DNA load ( r = 0.508, P = 0.008), as well as between the frequency of Th17 cells and serum alanine aminotransferase level ( r = 0.516, P = 0.007). The deletion of Treg cells significantly enhanced both Th17 and Tc17 cell development in PBMCs following recombinant HBV core antigen stimulation. Conclusions: Our data indicate a clear inverse relationship between Th17 cells and Treg cells and that Treg cells can inhibit Th17 cell development in CHB patients.
ACCESSION #
96716101

 

Related Articles

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics