TITLE

Synthesis of β-alanine from l-aspartate using l-aspartate-α-decarboxylase from Corynebacterium glutamicum

AUTHOR(S)
Shen, Yan; Zhao, Lianzhen; Li, Youran; Zhang, Liang; Shi, Guiyang
PUB. DATE
August 2014
SOURCE
Biotechnology Letters;Aug2014, Vol. 36 Issue 8, p1681
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
β-Alanine is mainly produced by chemical methods in current industrial processes. Here, panD from Corynebacterium glutamicum encoding l-aspartate-α-decarboxylase (ADC) was cloned and expressed in Escherichia coli BL21(DE3). ADC catalyzes the α-decarboxylation of l-aspartate to β-alanine. The purified ADC was optimal at 55 °C and pH 6 with excellent stability at 16-37 °C and pH 4-7. A pH-stat directed, fed-batch feeding strategy was developed for enzymatic synthesis of β-alanine to keep the pH value within 6-7.2 and thus attenuate substrate inhibition. A maximum conversion of 97.2 % was obtained with an initial 5 g l-aspartate/l and another three feedings of 0.5 % (w/v) l-aspartate at 8 h intervals. The final β-alanine concentration was 12.85 g/l after 36 h. This is the first study concerning the enzymatic production of β-alanine by using ADC.
ACCESSION #
96857845

 

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