Expression and evaluation of enzymes required for the hydrolysis of galactomannan

Malherbe, A.; Rose, S.; Viljoen-Bloom, M.; Zyl, W.
August 2014
Journal of Industrial Microbiology & Biotechnology;Aug2014, Vol. 41 Issue 8, p1201
Academic Journal
The cost-effective production of bioethanol from lignocellulose requires the complete conversion of plant biomass, which contains up to 30 % mannan. To ensure utilisation of galactomannan during consolidated bioprocessing, heterologous production of mannan-degrading enzymes in fungal hosts was explored. The Aspergillus aculeatus endo-β-mannanase ( Man1) and Talaromyces emersonii α-galactosidase ( Agal) genes were expressed in Saccharomyces cerevisiae Y294, and the Aspergillus niger β-mannosidase ( cMndA) and synthetic Cellvibrio mixtus β-mannosidase ( Man5A) genes in A. niger. Maximum enzyme activity for Man1 (374 nkat ml, pH 5.47), Agal (135 nkat ml, pH 2.37), cMndA (12 nkat ml, pH 3.40) and Man5A (8 nkat ml, pH 3.40) was observed between 60 and 70 °C. Co-expression of the Man1 and Agal genes in S. cerevisiae Y294[Agal-Man1] reduced the extracellular activity relative to individual expression of the respective genes. However, the combined action of crude Man1, Agal and Man5A enzyme preparations significantly decreased the viscosity of galactomannan in locust bean gum, confirming hydrolysis thereof. Furthermore, when complemented with exogenous Man5A, S. cerevisiae Y294[Agal-Man1] produced 56 % of the theoretical ethanol yield, corresponding to a 66 % carbohydrate conversion, on 5 g l mannose and 10 g l locust bean gum.


Related Articles

  • Study on the saccharification conditions of fuel ethanol from corn straw. Zhang Min; Li Jinyang; Fu Haibin // Applied Mechanics & Materials;2014, Issue 672-674, p172 

    To study the saccharification conditions of cellulase on lignocellulose material, this paper used corn straw as the material to do research on the effect of cellulase. The hydrolysis factors, including reaction temperature, reaction time and cellulase concentration, were measured in order to...

  • Weedy lignocellulosic feedstock and microbial metabolic engineering: advancing the generation of 'Biofuel'. Chandel, Anuj K.; Singh, Om V. // Applied Microbiology & Biotechnology;Mar2011, Vol. 89 Issue 5, p1289 

    Lignocellulosic materials are the most abundant renewable organic resources (~200 billion tons annually) on earth that are readily available for conversion to ethanol and other value-added products, but they have not yet been tapped for the commercial production of fuel ethanol. The...

  • Survey of Genes Involved in Rice Secondary Cell Wall Formation Through a Co-Expression Network. Hirano, Ko; Aya, Koichiro; Morinaka, Yoichi; Nagamatsu, Shiro; Sato, Yutaka; Antonio, Baltazar A.; Namiki, Nobukazu; Nagamura, Yoshiaki; Matsuoka, Makoto // Plant & Cell Physiology;Nov2013, Vol. 54 Issue 11, p1803 

    The plant secondary cell wall is the major source of lignocellulosic biomass, a renewable energy resource that can be used for bioethanol production. To comprehensively identify transcription factors (TFs), glycosyltransferase (GT) and glycosyl hydrolase (GH) involved in secondary cell wall...

  • Multiscale modelling of biomass pretreatment for biofuels production. Hosseini, Seyed Ali; Shah, Nilay // Chemical Engineering Research & Design: Transactions of the Inst;Sep2009, Vol. 87 Issue 9, p1251 

    Current fuel ethanol research deals with process engineering trends for improving the efficiency of bioethanol production. Pretreatment has been viewed as one of the most expensive and energy intensive processing steps in the lignocellulosic biomass-to-fermentable sugars conversion process. The...

  • Ethanol Production from Hydrothermal Pretreated Empty Fruit Bunches. Aisya, M. S. Siti; Petchpradab, Pacharakamol; Yoshimitsu Uemura; Yusup, S. Suzana; Kanna, Machi; Yoshimitsu Matsumura // Advanced Materials Research;2014, Vol. 917, p80 

    Separate hydrolysis and fermentation (SHF) is the common process in producing ethanol from lignocellulosic biomass. Nowadays, simultaneous saccharification and fermentation (SSF) process has been seen as potential process for producing ethanol with shortens process time with higher yield of...

  • Effect of lignin content of lignocellulosic material on hydrolysis efficiency. Raud, M.; Tutt, M.; Olt, J.; Kikas, T. // Agronomy Research;2015, Vol. 13 Issue 2, p405 

    Lignocellulosic material is the most promising feedstock for bioethanol production; however, due to the varying physicochemical characteristics of different biomasses, it is necessary to select a biomass with a composition suitable for bioethanol production. For this purpose several different...

  • A Constitutive Expression System for Cellulase Secretion in Escherichia coli and Its Use in Bioethanol Production. Munjal, Neha; Jawed, Kamran; Wajid, Saima; Yazdani, Syed Shams // PLoS ONE;Mar2015, Vol. 10 Issue 3, p1 

    The production of biofuels from lignocellulosic biomass appears to be attractive and viable due to the abundance and availability of this biomass. The hydrolysis of this biomass, however, is challenging because of the complex lignocellulosic structure. The ability to produce hydrolytic cellulase...

  • A comprehensive ligninolytic pre-treatment approach from lignocellulose green biotechnology to produce bio-ethanol. Asgher, Muhammad; Bashir, Fareeha; Nasir Iqbal, Hafız Muhammad // Chemical Engineering Research & Design: Transactions of the Inst;Aug2014, Vol. 92 Issue 8, p1571 

    In the present study, we developed a novel ligninolytic enzymes based pre-treatment method for lignocellulosic wheat straw to depolymerize lignin and expose the cellulose polymers to produce bio-ethanol. Wheat straw was pre-treated with ligninolytic enzymes extract produced from Ganoderma...

  • Bioconversion of Renewable Bioresources and Agricultural by-Products into Bioethanol. Ncibi, Mohamed Chaker // Recent Patents on Chemical Engineering;Nov2010, Vol. 3 Issue 3, p165 

    A review of the production technologies of bioethanol, an eco-friendly automotive fuel, from non-edible and renewable lignocellulosic bioresources and agricultural wastes is presented, based on the recently developed patents and research works. The different technologies such as ammonia...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics