TITLE

Development of species-specific quantitative real-time PCR primers for detecting anginosus group streptococci based on the rpoB

AUTHOR(S)
Park, Soon-Nang; Lim, Yun; Kook, Joong-Ki
PUB. DATE
September 2014
SOURCE
Archives of Microbiology;Sep2014, Vol. 196 Issue 9, p661
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
In this study, we introduced species-specific quantitative real-time PCR (qPCR) primers designed based on a DNA-dependent RNA polymerase beta-subunit gene for detecting anginosus group streptococci (AGS), Streptococcus anginosus, S. constellatus, and S. intermedius. The specificity of the qPCR primers was confirmed by conventional PCR with the genomic DNAs of 76 strains regarding 44 bacterial species including the type strain for the target species. The standard curves revealed the lower detection limits of these species-specific qPCR primers was 40 fg at below a cycle threshold (C) value of 35. These results suggest that AGS species-specific qPCR primers are suitable for applications in epidemiological studies associated with infectious diseases related to AGS.
ACCESSION #
97524228

 

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