Dengue Virus Neutralizing Antibody Levels Associated with Protection from Infection in Thai Cluster Studies

Buddhari, Darunee; Aldstadt, Jared; Endy, Timothy P.; Srikiatkhachorn, Anon; Thaisomboonsuk, Butsaya; Klungthong, Chonticha; Nisalak, Ananda; Khuntirat, Benjawan; Jarman, Richard G.; Fernandez, Stefan; Thomas, Stephen J.; Scott, Thomas W.; Rothman, Alan L.; Yoon, In-Kyu
October 2014
PLoS Neglected Tropical Diseases;Oct2014, Vol. 8 Issue 10, p1
Academic Journal
Background: Long-term homologous and temporary heterologous protection from dengue virus (DENV) infection may be mediated by neutralizing antibodies. However, neutralizing antibody titers (NTs) have not been clearly associated with protection from infection. Methodology/Principal Findings: Data from two geographic cluster studies conducted in Kamphaeng Phet, Thailand were used for this analysis. In the first study (2004–2007), cluster investigations of 100-meter radius were triggered by DENV-infected index cases from a concurrent prospective cohort. Subjects between 6 months and 15 years old were evaluated for DENV infection at days 0 and 15 by DENV PCR and IgM ELISA. In the second study (2009–2012), clusters of 200-meter radius were triggered by DENV-infected index cases admitted to the provincial hospital. Subjects of any age ≥6 months were evaluated for DENV infection at days 0 and 14. In both studies, subjects who were DENV PCR positive at day 14/15 were considered to have been “susceptible” on day 0. Comparison subjects from houses in which someone had documented DENV infection, but the subject remained DENV negative at days 0 and 14/15, were considered “non-susceptible.” Day 0 samples were presumed to be from just before virus exposure, and underwent plaque reduction neutralization testing (PRNT). Seventeen “susceptible” (six DENV-1, five DENV-2, and six DENV-4), and 32 “non-susceptible” (13 exposed to DENV-1, 10 DENV-2, and 9 DENV-4) subjects were evaluated. Comparing subjects exposed to the same serotype, receiver operating characteristic (ROC) curves identified homotypic PRNT titers of 11, 323 and 16 for DENV-1, -2 and -4, respectively, to differentiate “susceptible” from “non-susceptible” subjects. Conclusions/Significance: PRNT titers were associated with protection from infection by DENV-1, -2 and -4. Protective NTs appeared to be serotype-dependent and may be higher for DENV-2 than other serotypes. These findings are relevant for both dengue epidemiology studies and vaccine development efforts.


Related Articles

  • The performance of the SD BIOLINE Dengue DUO® rapid immunochromatographic test kit for the detection of NS1 antigen, IgM and IgG antibodies during a dengue type 1 epidemic in Jamaica. Vickers, Ivan E.; Harvey, Kevin M.; Brown, Michelle G.; Nelson, Kereann; DuCasse, Marion Bullock; Lindo, John F. // Journal of Biomedical Science;Jul2015, Vol. 22 Issue 1, p1 

    Background: Dengue is an important mosquito-borne viral infection that affects millions of persons worldwide. Early diagnosis is necessary to effect appropriate management and decrease mortality. Immunochromatographic tests are advantageous in producing dengue test results within 30 min but...

  • Enzyme-linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood. Tran, Thanh Nga T; de Vries, Peter J; Lan Phuong Hoang; Phan, Giao T; Le, Hung Q; Tran, Binh Q; Vo, Chi Mai T; Nguyen, Nam V; Kager, Piet A; Nagelkerke, Nico; Groen, Jan // BMC Infectious Diseases;2006, Vol. 6 Issue 1, p13 

    Background: The reproducibilty of dengue IgM and IgG ELISA was studied in serum and filter paper blood spots from Vietnamese febrile patients. Methods: 781 pairs of acute (t0) and convalescent sera, obtained after three weeks (t3) and 161 corresponding pairs of filter paper blood spots were...

  • Multi-Country Evaluation of the Sensitivity and Specificity of Two Commercially-Available NS1 ELISA Assays for Dengue Diagnosis. Guzman, Maria G.; Jaenisch, Thomas; Gaczkowski, Roger; Hang, Vo Thi Ty; Sekaran, Shamala Devi; Kroeger, Axel; Vazquez, Susana; Ruiz, Didye; Martinez, Eric; Mercado, Juan C.; Balmaseda, Angel; Harris, Eva; Dimano, Efren; Leano, Prisca Susan A.; Yoksan, Sutee; Villegas, Elci; Benduzu, Herminia; Villalobos, Iris; Farrar, Jeremy; Simmons, Cameron P. // PLoS Neglected Tropical Diseases;Aug2010, Vol. 4 Issue 8, p1 

    Background: Early diagnosis of dengue can assist patient triage and management and prevent unnecessary treatments and interventions. Commercially available assays that detect the dengue virus protein NS1 in the plasma/serum of patients offers the possibility of early and rapid diagnosis....

  • Cross-reactivities between human IgMs and the four serotypes of dengue virus as probed with artificial homodimers of domain-III from the envelope proteins. Zidane, Nora; Dussart, Philippe; Bremand, Laetitia; Bedouelle, Hugues // BMC Infectious Diseases;2013, Vol. 13 Issue 1, p1 

    Background: Dengue fever is the most important vector-borne viral disease. Four serotypes of dengue virus, DENV1 to DENV4, coexist. Infection by one serotype elicits long-lasting immunity to that serotype but not the other three. Subsequent infection by a different serotype is a risk factor for...

  • Association of the −1082 G/A promoter polymorphism of interleukin-10 gene with the autoantibodies production in patients with rheumatoid arthritis. Nemec, Petr; Pavkova-Goldbergova, Monika; Gatterova, Jindra; Fojtik, Zdenek; Vasku, Anna; Soucek, Miroslav // Clinical Rheumatology;Aug2009, Vol. 28 Issue 8, p899 

    Interleukin-10 (IL-10) is an immunoregulatory cytokine, usually considered to mediate the downregulation of the inflammatory response in rheumatoid arthritis (RA). Some effects of IL-10 are not anti-inflammatory; for example, the activation of B cells to promote autoantibody production. Allelic...

  • Evaluation of Commercially Available Diagnostic Tests for the Detection of Dengue Virus NS1 Antigen and Anti-Dengue Virus IgM Antibody. Hunsperger, Elizabeth A.; Yoksan, Sutee; Buchy, Philippe; Nguyen, Vinh Chau; Sekaran, Shamala Devi; Enria, Delia A.; Vazquez, Susana; Cartozian, Elizabeth; Pelegrino, Jose L.; Artsob, Harvey; Guzman, Maria G.; Olliaro, Piero; Zwang, Julien; Guillerm, Martine; Kliks, Susie; Halstead, Scott; Peeling, Rosanna W.; Margolis, Harold S. // PLoS Neglected Tropical Diseases;Oct2014, Vol. 8 Issue 10, p1 

    Commercially available diagnostic test kits for detection of dengue virus (DENV) non-structural protein 1 (NS1) and anti-DENV IgM were evaluated for their sensitivity and specificity and other performance characteristics by a diagnostic laboratory network developed by World Health Organization...

  • Seroepidemiology of Asymptomatic Dengue Virus Infection in Jeddah, Saudi Arabia. Jamjoom, Ghazi A.; Azhar, Esam I.; Kao, Moujahid A.; Radadi, Raja M. // Virology: Research & Treatment;2016, Vol. 7, p1 

    BACKGROUND: Although virologically confirmed dengue fever has been recognized in Jeddah, Saudi Arabia, since 1994, causing yearly outbreaks, no proper seroepidemiologic studies on dengue virus have been conducted in this region. Such studies can define the extent of infection by this virus and...

  • Development and Standardization of ELISA as Pre-screen Test for the Potency Estimation during Commercial Production of Antisnake Venom Serum (ASVS). Kumar, Sunil; Kumar, Sandeep; Behl, Yash; Ravi Kumar Gupta // American Journal of Biomedical Sciences;2014, Vol. 6 Issue 1, p20 

    In vivo neutralization assay is the only accepted method for the potency testing of antisnake venom serum (ASVS), till today. Due to non-availability of any other methods, this assay is considered as gold standard for potency testing. However, it requires large number of animals. There is an...

  • Brucellosis and the Respiratory System. Pappas, Georgios; Bosilkovski, Mile; Akritidis, Nikolaos; Mastora, Maria; Krteva, Liliana; Tsianos, Epaminondas // Clinical Infectious Diseases;10/1/2003, Vol. 37 Issue 7, p95 

    Describes the involvement of the respiratory system in brucellosis in Balkan Peninsula. Accounts on the predominance of monocytic or lymphocytic infiltrates; Inhalation of the infectious aerosols particles; Use of enzyme-linked immunosorbent assay to detect immunoglobulin M antibodies.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics