TITLE

Carbon partitioning to the terpenoid biosynthetic pathway enables heterologous β-phellandrene production in Escherichia coli cultures

AUTHOR(S)
Formighieri, Cinzia; Melis, Anastasios
PUB. DATE
December 2014
SOURCE
Archives of Microbiology;Dec2014, Vol. 196 Issue 12, p853
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Escherichia coli was used as a microbial system for the heterologous synthesis of β-phellandrene, a monoterpene of plant origin with several potential commercial applications. Expression of Lavandula angustifolia β-phellandrene synthase ( PHLS), alone or in combination with Picea abies geranyl-diphosphate synthase in E. coli, resulted in no β-phellandrene accumulation, in sharp contrast to observations with PHLS-transformed cyanobacteria. Lack of β-phellandrene biosynthesis in E. coli was attributed to the limited endogenous carbon partitioning through the native 2- C-methylerythritol-4-phosphate (MEP) pathway. Heterologous co-expression of the mevalonic acid pathway, enhancing cellular carbon partitioning and flux toward the universal isoprenoid precursors, isopentenyl-diphosphate and dimethylallyl-diphosphate, was required to confer β-phellandrene production. Differences in endogenous carbon flux toward the synthesis of isoprenoids between photosynthetic ( Synechocystis) and non-photosynthetic bacteria ( E. coli) are discussed in terms of differences in the regulation of carbon partitioning through the MEP biosynthetic pathway in the two systems.
ACCESSION #
99455582

 

Related Articles

  • Mutations in Escherichia coli aceE and ribB Genes Allow Survival of Strains Defective in the First Step of the Isoprenoid Biosynthesis Pathway. Perez-Gil, Jordi; Uros, Eva Maria; Sauret-Güeto, Susanna; Lois, L. Maria; Kirby, James; Nishimoto, Minobu; Baidoo, Edward E. K.; Keasling, Jay D.; Boronat, Albert; Rodriguez-Concepcion, Manuel; McCutcheon, John // PLoS ONE;Aug2012, Vol. 7 Issue 8, Special section p1 

    A functional 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is required for isoprenoid biosynthesis and hence survival in Escherichia coli and most other bacteria. In the first two steps of the pathway, MEP is produced from the central metabolic intermediates pyruvate and glyceraldehyde...

  • Computational identification of gene over-expression targets for metabolic engineering of taxadiene production. Boghigian, Brett; Armando, John; Salas, Daniel; Pfeifer, Blaine // Applied Microbiology & Biotechnology;Mar2012, Vol. 93 Issue 5, p2063 

    Taxadiene is the first dedicated intermediate in the biosynthetic pathway of the anticancer compound Taxol. Recent studies have taken advantage of heterologous hosts to produce taxadiene and other isoprenoid compounds, and such ventures now offer research opportunities that take advantage of the...

  • Simultaneous production and partitioning of heterologous polyketide and isoprenoid natural products in an Escherichia coli two-phase bioprocess. Boghigian, Brett; Myint, Melissa; Wu, Jiequn; Pfeifer, Blaine // Journal of Industrial Microbiology & Biotechnology;Nov2011, Vol. 38 Issue 11, p1809 

    Natural products have long served as rich sources of drugs possessing a wide range of pharmacological activities. The discovery and development of natural product drug candidates is often hampered by the inability to efficiently scale and produce a molecule of interest, due to inherent qualities...

  • A novel fungal prenyl diphosphate synthase in the dimorphic zygomycete Mucor circinelloides. Velayos, Antonio; Fuentes-Vicente, Mónica; Aguilar-Elena, Raúl; Eslava, Arturo P.; Iturriaga, Enrique A. // Current Genetics;Jun2004, Vol. 45 Issue 6, p371 

    Two Mucor circinelloides structural genes involved in isoprenoid biosynthesis were isolated and characterised. The isoA gene encodes a typical eukaryotic farnesyl diphosphate synthase (EC 2.5.1.10), whereas the isoB gene deduced amino acid sequence shows similarity to fungal medium-chain prenyl...

  • Biogenic Hydrocarbons in the Atmospheric Boundary Layer: A Review. Judson, A.; Doesken, N. // Bulletin of the American Meteorological Society;Jul2000, Vol. 81 Issue 7, p1537 

    Provides insights into the state if the science concerning biosynthesis, transport and chemical transformation of hydrocarbons emitted by the terrestrial biosphere. Focus on isoprene, monoterpens and oxygenated hydrocarbons; Discussion of the physiological and environmental processes...

  • Study of Storage Periods of Culture Suspension of Escherichia coli. Kundan Kumar; I. N. Mishra; Sandhya Jha // International Journal of Pharmaceutical Sciences & Drug Research;Jan-Mar2013, Vol. 5 Issue 1, p36 

    Escherichia coli strain ATCC 8739 is an important strain of pharmaceutical and biotechnological industries. We studied the viability of culture suspension of Escherichia coli strain ATCC 8739 at 2 to 8°C up to 360 days in 0.9% w/v NaCl. The culture suspension containing 10000 cfu/ml used in...

  • Biosynthesis of isoprenoids via the non-mevalonate pathway. Eisenreich, W.; Bacher, A.; Arigoni, D.; Rohdich, F. // Cellular & Molecular Life Sciences;Jun2004, Vol. 61 Issue 12, p1401 

    The mevalonate pathway for the biosynthesis of the universal terpenoid precursors, isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), is known in considerable detail. Only recently, the existence of a second mevalonate-independent pathway for the biosynthesis of IPP and DMAPP...

  • Assessment of Quality of Camel Milk and Gariss, North Kordofan State, Sudan. Ahmed, Adam Ismail; Mohammed, Abdelrahman Ahmed; Faye, Bernard; Blanchard, Lucie; Bakheit, Sallam. A. // Research Journal of Animal & Veterinary Sciences;2010, Vol. 5, p18 

    The present study was conducted near Elobeid north Kordofan state, Sudan to assess the quality of camel milk and gariss. The total viable count of bacteria was measured accompanied by the enumeration of other flora on milk and gariss, then pathogencity of the milk and gariss study by presence of...

  • Phenotypic and Genotypic Characterization of Enteroaggregative Escherichia coli Strains Isolated From Diarrheic Children in Iran. Davoodabadi, Abolfazl; Abbaszadeh, Maryam; Oloomi, Mana; Bouzari, Saeid // Jundishapur Journal of Microbiology;Sep2015, Vol. 8 Issue 9, p1 

    Background: Several studies performed in developed and developing countries have identified enteroaggregative Escherichia coli (EAEC) as the emerging cause of pediatric diarrhea. Objectives: This study investigated the phenotypic and genetic characteristics of EAEC strains isolated from children...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics