TITLE

Activation of extracellular signal-regulated kinase mediates apoptosis induced by uropathogenic Escherichia coli toxins via nitric oxide synthase: protective role of heme oxygenase-1

AUTHOR(S)
Chen, Ming; Bao, Wenjie; Aizman, Roman; Huang, Ping; Aspevall, Olle; Gustafson, Lars E.; Ceccatelli, Sandra; Celsi, Gianni; Gustafsson, Lars E
PUB. DATE
July 2004
SOURCE
Journal of Infectious Diseases;7/1/2004, Vol. 190 Issue 1, p127
SOURCE TYPE
Academic Journal
DOC. TYPE
journal article
ABSTRACT
Pyelonephritis is a risk factor for renal tubular epithelial cell damage in children. The inter- and intracellular regulator nitric oxide (NO) plays a role in the modulation of cellular viability in urinary tract infections, but the role of the NO pathway in renal proximal tubular-cell death remains unclear. The present study demonstrates that, in renal epithelial cells undergoing death mediated by Escherichia coli strain ARD6 serotype O6K13H1 (O6), levels of the phosphorylated extracellular signal-regulated kinase (ERK) 1/2 and inducible NO synthase (iNOS) proteins are up-regulated, but levels of endothelial NO synthase are down-regulated. When NO synthase (NOS) activity is inhibited by the specific inhibitor of NOS or mitogen-activated protein kinase kinase, cells are prevented from death. Moreover, down-regulating protein 53 (p53) does not prevent the cells from dying, although p53 is up-regulated in O6-exposed cells. Up-regulation of heme oxygenase (HO)-1 by sodium nitroprusside or by the specific activator hemin inhibits cell death. In conclusion, the activation of ERK mediates O6 toxin-mediated renal cell death via induction of iNOS. Stimulation of HO-1 protects cells against death.
ACCESSION #
13432138

 

Related Articles

  • A novel target of mizoribine inhibiting mesangial cell proliferation: S phase kinase-associated protein 2. Liu, Shuxin; Xie, Yuansheng; Lv, Yang; Qin, Fufang; Fu, Bo; Shi, Suozhu; Yin, Zhong; Hong, Quan; Zhang, Xueguang; Wang, Jianzhong; Chang, Ming; Chen, Xiangmei // American Journal of Nephrology;2010, Vol. 32 Issue 5, p447 

    Background/aims: Mizoribine (MZR) can inhibit mesangial cell (MC) proliferation, but the mechanism remains unknown. In this study, we investigated the inhibitory effect of MZR on MC proliferation via a cell cycle regulatory protein-dependent mechanism.Methods: We...

  • TNF provokes cardiomyocyte apoptosis and cardiac remodeling through activation of multiple cell death pathways. Haudek, Sandra B.; Taffet, George E.; Schneider, Michael D.; Mann, Douglas L. // Journal of Clinical Investigation;Sep2007, Vol. 117 Issue 9, p2692 

    Transgenic mice with cardiac-restricted overexpression of secretable TNF (MHCsTNF) develop progressive LV wall thinning and dilation accompanied by an increase in cardiomyocyte apoptosis and a progressive loss of cytoprotective Bcl-2. To test whether cardiac-restricted overexpression of Bcl-2...

  • NADPH oxidase signal transduces angiotensin II in hepatic stellate cells and is critical in hepatic fibrosis. Bataller, Ramón; Scbwabe, Robert F.; Choi, Youkyung H.; Liu Yang; Yong Han Paik, Youkyung H.; Lindquist, Jeffrey; Ting Qian; Schoonhoven, Robert; Hagedorn, Curt H.; Lemasters, John J.; Brenner, David A.; Bataller, Ramon; Schwabe, Robert F; Yang, Liu; Paik, Yong Han; Qian, Ting // Journal of Clinical Investigation;Nov2003, Vol. 112 Issue 9, p1383 

    Angiotensin II (Ang II) is a pro-oxidant and fibrogenic cytokine. We investigated the role of NADPH oxidase in Ang II-induced effects in hepatic stellate cells (HSCs), a fibrogenic cell type. Human HSCs express mRNAs of key components of nonphagocytic NADPH oxidase. Ang II phosphorylated...

  • Prostaglandin E2 reduces radiation-induced epithelial apoptosis through a mechanism involving AKT activation and bax translocation. Tessner, Teresa G.; Muhale, Filipe; Riehl, Terrence E.; Anant, Shrikant; Stenson, William F. // Journal of Clinical Investigation;Dec2004, Vol. 114 Issue 11, p1676 

    Prostaglandin E2 (PGE2) synthesis modulates the response to radiation injury in the mouse intestinal epithelium through effects on crypt survival and apoptosis; however, the downstream signaling events have not been elucidated. WT mice receiving 16,16-dimethyl PGE2 (dmPGE2) had fewer apoptotic...

  • Nicotine exposure and bronchial epithelial cell nicotinic acetylcholine receptor expression in the pathogenesis of lung cancer. Minna, John D. // Journal of Clinical Investigation;1/1/2003, Vol. 111 Issue 1, p31 

    Comments on a research on the role of neuronal nicotinic acetylcholine receptors (nAchRs) in lung cancer. Mechanism by which nicotine could block apoptosis; Cellular targets and the role of other nAchR subunit types; Biochemical events generated after nicotine interacts with its receptor.

  • The 1996 Albert Lasker Medical Research Awards. Signal transduction using nitric oxide and cyclic guanosine monophosphate. Murad, F // JAMA: Journal of the American Medical Association;10/9/96, Vol. 276 Issue 14, p1189 

    Understanding of the formation and biological actions of nitric oxide (NO) has grown extensively during the past 2 decades. Through our discoveries of the biological effects of NO and nitrovasodilators on cyclic guanosine monophosphate (GMP) and our discoveries of the biochemical mechanisms of...

  • Role of heme oxygenase-1 in morphine-modulated apoptosis and migration of macrophages. Patel, Kalpesh; Bhaskaran, Madhu; Dani, Dhimant; Reddy, Krishna; Singhal, Pravin C. // Journal of Infectious Diseases;1/1/2003, Vol. 187 Issue 1, p47 

    We examined the role of heme oxygenase (HO)-1 in morphine-induced decrease in macrophage migration. Morphine promoted expression of HO-1 in murine macrophages. Morphine-receiving mice (MRCs) showed decreased (P<.001) macrophage migration and increased (P<.001) occurrence of macrophage apoptosis....

  • Characterization of glycosaminoglycans in tubular epithelial cells: calcium oxalate and oxalate ions effects. Borges, Fernanda T.; Michelacci, Yara M.; Aguiar, Jair A. K.; Dalboni, Maria A.; Garófalo, Andrezza S.; Schor, Nestor; Garófalo, Andrezza S // Kidney International;Oct2005, Vol. 68 Issue 4, p1630 

    Background: The interaction between tubular epithelial cells and calcium oxalate crystals or oxalate ions is a very precarious event in the lithogenesis. Urine contains ions, glycoproteins and glycosaminoglycans that inhibit the crystallization process and may protect the kidney...

  • Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury. Luedde, Tom; Assrnus, Ulnke; Wüstefeld, Torsten; Vilsendori, Andreas Meyer Zu; Roskams, Tania; Schmidt-supprian, Mark; Rajewsky, Klaus; Brenner, DavidA.; Manns, Michael P.; Pasparakis, Manolis; Trautwein, Christian; Assmus, Ulrike; Wüstefeld, Torsten; Meyer zu Vilsendorf, Andreas; Brenner, David A // Journal of Clinical Investigation;Apr2005, Vol. 115 Issue 4, p849 

    The inhibitor of NF-kappaB (I-kappaB) kinase (IKK) complex consists of 3 subunits, IKK1, IKK2, and NF-kappaB essential modulator (NEMO), and is involved in the activation of NF-kappaB by various stimuli. IKK2 or NEMO constitutive knockout mice die during embryogenesis as a result of massive...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics